Page 30 - SaxoCell Annual Report22/23
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Efficient transgene expression of AAV vectors in primary NK cells was demonstrated for the first
time. Different AAV serotypes and capsid variants were compared and optimized, with
approximately 80% eGFP transgene expression. Initial AAV-CAR vectors in NK cells showed
increased cytotoxicity. Further characterization and optimization steps, including intracellular
processing of AAV vectors, are in progress.
Transduction of primary human natural killer (NK) cells with adeno-associated viral (AAV) vectors. AAV vectors could be
successfully and efficiently used for transduction and transgene expression of human primary NK cells. The basic transgene
expression level of AAV vectors is very low and highly donor-dependent (see AAV vector). The addition of an activation mix
increases the level of transgene expression to ˜80%, donor-independent (see AAV vector + activation mix).
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