Page 50 - SaxoCell Annual Report22/23

OMICS

The mission of SaxoCellOMICS is to provide a common platform incorporating state-of-the-art
cellular and molecular measurement techniques, data processing as well as interpretation. This
platform will support the development and manufacturing of gene and cell therapeutics by
providing efficient methods for monitoring therapies, identifying mechanisms of action and novel
targets, and developing quality criteria for manufacturing and predictive biomarker development.
By working closely with other SaxoCell partners, SaxoCellOMICS contributes to strengthening the
competitiveness of companies in the SaxoCell region.

Project lead: Kristin Reiche & Ezio Bonifacio

Partners: Fraunhofer IZI, TUD Dresden University of Technology, Leipzig University, ecSeq GmbH

In 2022/23, a comprehensive list of relevant methods and technologies was compiled and made
available to all SaxoCell partners via the website in the members' area. This process was carried
out in collaboration with the SaxoCellOMICS partners and the SaxoCellHub.

In addition, recommendations and standard operating procedures (SOPs) for sample collection
and processing were developed and harmonized. Existing workflows such as flow cytometry and
transcriptome sequencing were used as templates. Harmonization will be performed between the
TUD and IZI production sites in collaboration with SaxoCellCLINICS. In addition, SOPs and
guidelines for data generation and analysis of selected assays are being developed.

Another important step was the preparation of a description of available experimental workflows
and analysis types as well as a cost matrix. This matrix will provide guidance and costing for
SaxoCell projects.

A major focus of the project is to coordinate and harmonize the methods available at the different
sites, e.g., flow cytometry (FACS) or RNAseq, by performing interlaboratory comparisons between
SaxoCellOMICS partners to determine and, if necessary, improve the agreement of measurements
between sites. Planning for these interlaboratory comparisons has already begun, and samples
have been identified and methods for sample processing established.
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